中文名称 | 5-羧基四甲基罗丹明琥珀酰亚胺酯 |
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英文名称 | 5-Carboxytetramethylrhodamine succinimidyl ester(5-TAMRASE) | 5-TAMRA, SE |
CAS号 | 150810-68-7 |
分子式 | C29H25N3O7 |
分子量 | 527.53 |
外观 | Red solid |
储存条件 | Dry, dark and at 0 - 4 ℃ for short term (days to weeks) or -20 ℃ for long term (months to years). |
5-Carboxytetramethylrhodamine succinimidyl ester (5-TAMRA-SE) is an amine-reactive fluorescent probe. It has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays. It quenches lucifer yellow CH fluorescence by greater than 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity. It has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including the antibiotic ampicillin , nucleotide diphosphate uridine-5’-diphosphate , and the progesterone receptor antagonist RU486. 5-TAMRA-SE displays excitation maxima ranging from 540 to 560 nm and an emission maximum of 580 nm.
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