英文名称 | Coelenterazine |
---|---|
中文名称 | 腔肠素 |
CAS号 | 55779-48-1 |
分子式 | C26H21N3O3 |
分子量 | 423.46 |
外观 | Yellow to brown solid |
储存条件 | Dry, dark and at 0 - 4 ℃ for short term (days to weeks) or -20 ℃ for long term (months to years). |
英文名称 | Coelenterazine |
---|---|
中文名称 | 腔肠素 |
CAS号 | 55779-48-1 |
分子式 | C26H21N3O3 |
分子量 | 423.46 |
外观 | Yellow to brown solid |
储存条件 | Dry, dark and at 0 - 4 ℃ for short term (days to weeks) or -20 ℃ for long term (months to years). |
Coelenterazine, a luciferin which is a light-emitting molecule, is a luminescent enzyme substrate that is used for monitoring reporter genes in BRET, ELISA and HTS techniques. It is a cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescent probe for the superoxide anion. Coelenterazine can also be used for detecting changes in intracellular Ca2+ in cells that have been transfected with apoaequorin cDNA. Coelenterazine also acts as a powerful antioxidant.